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SERS Microscopy: Nanoparticle Probes and Biomedical Applications
Author(s) -
Schlücker Sebastian
Publication year - 2009
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200900119
Subject(s) - biomolecule , nanotechnology , microscopy , nanoparticle , raman scattering , raman spectroscopy , materials science , chemistry , optics , physics
Microspectroscopic imaging: Surface‐enhanced Raman scattering (SERS) microscopy is a novel method of vibrational microspectroscopic imaging for the selective detection of biomolecules in targeted research. This review summarizes current designs of nanoparticle‐based SERS probes (see figure) and highlights first biomedical applications of SERS microscopy for protein localization ex and in vivo.Surface‐enhanced Raman scattering (SERS) microscopy is a novel method of vibrational microspectroscopic imaging for the selective detection of biomolecules in targeted research. This technique combines the advantages of biofunctionalized metal nanoparticles and Raman microspectroscopy for visualizing and quantifying the distribution of target molecules such as proteins in cells and tissues. Advantages of SERS over existing labeling approaches include the tremendous multiplexing capacity, quantification using the characteristic SERS signatures and high photostability. This review summarizes current designs of nanoparticle‐based SERS probes and highlights first biomedical applications of SERS microscopy for protein localization ex and in vivo.