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Oxygen Microscopy by Two‐Photon‐Excited Phosphorescence
Author(s) -
Finikova Olga S.,
Lebedev Artem Y.,
Aprelev Alexey,
Troxler Thomas,
Gao Feng,
Garnacho Carmen,
Muro Silvia,
Hochstrasser Robin M.,
Vinogradov Sergei A.
Publication year - 2008
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200800296
Subject(s) - phosphorescence , microscopy , excited state , nanoprobe , oxygen , two photon excitation microscopy , absorption (acoustics) , chemistry , optical sectioning , excitation , analytical chemistry (journal) , materials science , photochemistry , optics , atomic physics , fluorescence , physics , organic chemistry , quantum mechanics , chromatography
High‐resolution images of oxygen distributions in microheterogeneous samples are obtained by two‐photon laser scanning microscopy (2P LSM), using a newly developed dendritic nanoprobe with internally enhanced two‐photon absorption (2PA) cross‐section. In this probe, energy is harvested by a 2PA antenna, which passes excitation onto a phosphorescent metalloporphyrin via intramolecular energy transfer. The 2P LSM allows sectioning of oxygen gradients with near diffraction‐limited resolution, and lifetime‐based acquisition eliminates dependence on the local probe concentration. The technique is validated on objects with a priori known oxygen distributions and applied to imaging of pO 2 in cells.