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Cover Picture: Two‐Focus Fluorescence Correlation Spectroscopy: A New Tool for Accurate and Absolute Diffusion Measurements (ChemPhysChem 3/2007)
Author(s) -
Dertinger Thomas,
Pacheco Victor,
von der Hocht Iris,
Hartmann Rudolf,
Gregor Ingo,
Enderlein Jörg
Publication year - 2007
Publication title -
chemphyschem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200790004
Subject(s) - fluorescence correlation spectroscopy , fluorescence , autocorrelation , spectroscopy , diffusion , focus (optics) , fluorescence spectroscopy , chemistry , fluorescence cross correlation spectroscopy , cover (algebra) , optics , physics , mathematics , statistics , quantum mechanics , thermodynamics , mechanical engineering , engineering
The cover picture shows the two focal regions of a two‐focus fluorescence correlation spectroscopy set‐up with molecules diffusing through these regions. The depicted curves are the fluorescence autocorrelation curves for the first and the second focus, respectively, as well as the fluorescence cross‐correlation between both foci. In their Article on page 433 Dertinger et al. introduce an external ruler into a fluorescence correlation measurement by having two focal regions with a fixed and well‐defined distance. This allows for absolute and precise determination of molecular diffusion.