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Single DNA Molecule Isolation and Trapping in a Microfluidic Device
Author(s) -
Kumemura Momoko,
Collard Dominique,
Yamahata Christophe,
Sakaki Naoyoshi,
Hashiguchi Gen,
Fujita Hiroyuki
Publication year - 2007
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200700268
Subject(s) - microfluidics , trapping , nanotechnology , molecule , dna , materials science , isolation (microbiology) , optical tweezers , chemistry , biology , physics , optics , bioinformatics , ecology , biochemistry , organic chemistry
This paper presents a systematic method to isolate and trap long single DNA segments between integrated electrodes in a microfluidic environment. Double stranded λ ‐DNA molecules are introduced in a microchip and are isolated by electrophoretic force through microfluidic channels. Downstream, each individual molecule is extended and oriented by ac dielectrophoresis (900 kHz, 1 MV m −1 ) and anchored between aluminium electrodes. With a proper design, a long DNA segment (up to 10 μm) can be instantly captured in stretched conformation, opening way for further assays.