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Sizing Trinucleotide Repeat Sequences by Single‐Molecule Analysis of Fluorescence Brightness
Author(s) -
Schlapak Robert,
Kinns Helen,
Wechselberger Christian,
Hesse Jan,
Howorka Stefan
Publication year - 2007
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200700163
Subject(s) - fluorescence , brightness , dna , fluorescence microscope , cytidine , biophysics , molecule , huntingtin , single molecule experiment , sequence (biology) , base pair , dna sequencing , chemistry , biology , genetics , physics , optics , biochemistry , gene , organic chemistry , mutant , enzyme
Sequence information of individual DNA strands is determined by the frequency with which a specific base occurs in a strand. Using huntingtin as a model system (see picture), the number of CAG repeats is obtained by incorporating fluorescent cytidine and analyzing the accumulated brightness of DNA strands by single‐molecule fluorescence microscopy.