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Specific Vectorial Immobilization of Oligonucleotide‐Modified Yeast Cytochrome c on Carbon Nanotubes
Author(s) -
Heering Hendrik A.,
Williams Keith A.,
de Vries Simon,
Dekker Cees
Publication year - 2006
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200600108
Subject(s) - oligonucleotide , carbon nanotube , yeast , chemistry , carbon fibers , nanotechnology , chemical engineering , dna , materials science , biochemistry , composite number , engineering , composite material
Iso‐1‐cytochrome c from the yeast Saccharomyces cerevisiae (YCC) contains a surface cysteine residue, Cys102, that is located opposite to the lysine‐rich side containing the exposed heme edge, which is the docking site for enzymes. Site‐specific vectorial immobilization of YCC via Cys102 on single‐walled carbon nanotubes (SWNT) thus provides a selective interface between nanoscopic electronic devices and complex enzymes. We have achieved this by modification of Cys102 with an oligonucleotide (dT 18 ). Atomic force microscopy, fluorescence imaging, and cyclic voltammetry show the specific adsorption of YCC, modified with dT 18 , on the SWNT sidewall with retention of its native properties. Pretreatment of the SWNT with Triton‐X405 blocks the nonspecific binding of untreated YCC but does not interfere with binding of the oligonucleotide‐modified YCC.