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Quantum‐Dot‐Labeled DNA Probes for Fluorescence In Situ Hybridization (FISH) in the Microorganism Escherichia coli
Author(s) -
Wu ShengMei,
Zhao Xiang,
Zhang ZhiLing,
Xie HaiYan,
Tian ZhiQuan,
Peng Jun,
Lu ZheXue,
Pang DaiWen,
Xie ZhiXiong
Publication year - 2006
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200500608
Subject(s) - fluorescence , quantum dot , dna , in situ , hybridization probe , chemistry , biosensor , escherichia coli , fluorescence in situ hybridization , absorption (acoustics) , dna–dna hybridization , nanotechnology , biophysics , materials science , biochemistry , gene , biology , optics , physics , organic chemistry , composite material , chromosome
Semiconductor quantum dots (QDs) as a kind of nonisotopic biological labeling material have many unique fluorescent properties relative to conventional organic dyes and fluorescent proteins, such as composition‐ and size‐dependent absorption and emission, a broad absorption spectrum, photostability, and single‐dot sensitivity. These properties make them a promising stable and sensitive label, which can be used for long‐term fluorescent tracking and subcellular location of genes and proteins. Here, a simple approach for the construction of QD‐labeled DNA probes was developed by attaching thiol‐ssDNA to QDs via a metal–thiol bond. The as‐prepared QD‐labeled DNA probes had high dispersivity, bioactivity, and specificity for hybridization. Based on such a kind of probe with a sequence complementary to multiple clone sites in plasmid pUC18, fluorescence in situ hybridization of the tiny bacterium Escherichia coli has been realized for the first time.