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Cover Picture: Direct Visualization of Lipid Phase Segregation in Single Lipid Bilayers with Coherent Anti‐Stokes Raman Scattering Microscopy (ChemPhysChem 1/2005)
Author(s) -
Potma Eric O.,
Xie X. Sunney
Publication year - 2005
Publication title -
chemphyschem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200490060
Subject(s) - lipid bilayer , microscopy , raman spectroscopy , raman scattering , phase (matter) , vesicle , lipid bilayer phase behavior , materials science , model lipid bilayer , chemistry , crystallography , optics , membrane , physics , biochemistry , organic chemistry
The cover picture shows lipid phase segregation in a single giant unilamellar vesicle (GUV) visualized with coherent anti‐Stokes Raman scattering (CARS) microscopy. Phase segregation can be clearly observed, as is evidenced by the CARS image of the deuterated lipid (red) overlaid for clarity with the image of the undeuterated lipid (green). The high sensitivity of CARS microscopy allows visualization of the GUV single lipid bilayer when the Raman shift is tuned into the frequency of the symmetric stretching vibrational mode of CH 2 , which is abundant in lipid molecules. X. S. Xie et al. show in their Communication on page 77 that CARS microscopy opens up possibilities for intracellular studies of lipid domains.