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Direct Visualization of Lipid Phase Segregation in Single Lipid Bilayers with Coherent Anti‐Stokes Raman Scattering Microscopy
Author(s) -
Potma Eric O.,
Xie X. Sunney
Publication year - 2005
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200400390
Subject(s) - raman scattering , vesicle , raman spectroscopy , microscopy , coherent anti stokes raman spectroscopy , chemistry , lipid bilayer , phase (matter) , scattering , materials science , analytical chemistry (journal) , molecular physics , optics , membrane , physics , chromatography , organic chemistry , biochemistry
Imaging of giant vesicles : Coherent anti‐Stokes Raman scattering (CARS) microscopy is used for imaging of giant vesicles of binary mixtures of lipids. The vibrational selectivity of the CARS microscope allows determination of molecules based on differences in the vibrational modes. The picture shows Raman and CARS spectra as well as CARS images of a deuterated giant unilamellar vesicle.