Optical Saturation in Fluorescence Correlation Spectroscopy under Continuous‐Wave and Pulsed Excitation | Zendy

Gregor Ingo | Zendy; Patra Digambara | Zendy; Enderlein Jörg | Zendy

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Optical Saturation in Fluorescence Correlation Spectroscopy under Continuous‐Wave and Pulsed Excitation

Author(s) -

Gregor Ingo,

Patra Digambara,

Enderlein Jörg

Publication year - 2005

Publication title -

chemphyschem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.016

H-Index - 140

eISSN - 1439-7641

pISSN - 1439-4235

DOI - 10.1002/cphc.200400319

Subject(s) - fluorescence correlation spectroscopy , excitation , saturation (graph theory) , fluorescence , fluorescence cross correlation spectroscopy , spectroscopy , fluorescence spectroscopy , chemistry , diffusion , molecular physics , atomic physics , materials science , analytical chemistry (journal) , nuclear magnetic resonance , optics , physics , mathematics , chromatography , combinatorics , quantum mechanics , thermodynamics

A detailed theoretical and experimental study of the dependence of fluorescence correlation measurements on optical excitation power due to optical saturation effects is presented. It is shown that the sensitivity of a fluorescence correlation measurement on excitation power becomes increasingly stronger for decreasing excitation power. This makes exact measurements of diffusion coefficients with fluorescence correlation spectroscopy rather difficult. A strong difference of this behavior for continuous‐wave and pulsed excitation is found.

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