Premium
Optical Saturation in Fluorescence Correlation Spectroscopy under Continuous‐Wave and Pulsed Excitation
Author(s) -
Gregor Ingo,
Patra Digambara,
Enderlein Jörg
Publication year - 2005
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200400319
Subject(s) - fluorescence correlation spectroscopy , excitation , saturation (graph theory) , fluorescence , fluorescence cross correlation spectroscopy , spectroscopy , fluorescence spectroscopy , chemistry , diffusion , molecular physics , atomic physics , materials science , analytical chemistry (journal) , nuclear magnetic resonance , optics , physics , mathematics , chromatography , combinatorics , quantum mechanics , thermodynamics
Abstract A detailed theoretical and experimental study of the dependence of fluorescence correlation measurements on optical excitation power due to optical saturation effects is presented. It is shown that the sensitivity of a fluorescence correlation measurement on excitation power becomes increasingly stronger for decreasing excitation power. This makes exact measurements of diffusion coefficients with fluorescence correlation spectroscopy rather difficult. A strong difference of this behavior for continuous‐wave and pulsed excitation is found.