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Three‐Color Single‐Molecule Fluorescence Resonance Energy Transfer
Author(s) -
Clamme JeanPierre,
Deniz Ashok A.
Publication year - 2005
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200400261
Subject(s) - förster resonance energy transfer , fluorescence , molecule , chemistry , resonance (particle physics) , energy transfer , fluorescence in the life sciences , chemical physics , optics , physics , atomic physics , organic chemistry
A molecular ruler: A three‐color single‐molecule fluorescence resonance energy transfer (FRET) method is presented (see Figure inset), which can be used to simultaneously measure multiple molecular distance changes during molecular conformational changes and binding. The ability to directly study conformational subpopulations in a mixture of molecules with different interdye distances is highlighted by the well‐separated peaks in the two‐ dimensional histogram in the Figure.