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Photon Antibunching Proves Emission from a Single Subunit in the Autofluorescent Protein DsRed
Author(s) -
SánchezMosteiro Gabriel,
Koopman Majolein,
van Dijk Erik M. H. P.,
Hernando Jordi,
van Hulst Niek F.,
GarcíaParajó María F.
Publication year - 2004
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200400216
Subject(s) - tetramer , intermolecular force , fluorescence , photon antibunching , quantum , protein subunit , chemical physics , photon , chemistry , molecular physics , physics , optics , quantum mechanics , nuclear magnetic resonance , molecule , biochemistry , gene , enzyme
Many colors red: Photon antibunching provides a unique way of revealing the single quantum character in a multichromophoric system. Here, it is demonstrated that the fluorescence emission in the DsRed tetramer (see picture) proceeds dominantly from one emitter at a time. This approach has equal potential for the investigation of conformational fluctuations and intermolecular interactions of complex (bio)systems at room temperature.