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No Label Required: Protein Binding at Membrane Interfaces Visualized through Colloid Phase Transitions
Author(s) -
Steinem Claudia,
Janshoff Andreas
Publication year - 2004
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200400092
Subject(s) - colloid , membrane , phase transition , membrane protein , chemistry , phase (matter) , biophysics , nanotechnology , chemical physics , materials science , biochemistry , biology , physics , organic chemistry , quantum mechanics
Colloid science meets biology. A new method has been introduced to visualize the interaction of proteins (purple) with membrane‐coated silica beads (surface shown in orange) by observing a phase transition from a condensed to a dispersed phase. Changes in the radial pair distribution function provide a sensitive means to quantify the interaction of proteins with membrane‐confined receptors.