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Dual‐Color Fluorescence Cross‐Correlation Spectroscopy Using Single Laser Wavelength Excitation
Author(s) -
Hwang Ling Chin,
Wohland Thorsten
Publication year - 2004
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200301057
Subject(s) - förster resonance energy transfer , spectroscopy , fluorescence , fluorescence correlation spectroscopy , fluorescence cross correlation spectroscopy , excitation , chemistry , fluorescence spectroscopy , quantum dot , laser induced fluorescence , laser , analytical chemistry (journal) , molecular physics , optics , materials science , optoelectronics , physics , quantum mechanics , chromatography
Spotlight on interactions: The authors show that single wavelength excitation fluorescence cross‐correlation spectroscopy (SW‐FCCS) is possible using only one laser beam to excite a combination of labels, in work performed with studied FRET (fluorescence resonance energy transfer) dyes and quantum dots. They demonstrate that equilibrium binding and stoichiometry can easily be determined (see graphic; QR = quantum red dye, BF = fluorescein‐labeled biotin). SW‐FCCS is a promising tool for high‐throughput screening applications as well as for the measurement of molecular interactions.