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Extraction of Kinetic Information from Single‐Molecule Experiments
Author(s) -
Vlad Marcel O.,
Ross John
Publication year - 2004
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200300991
Subject(s) - intramolecular force , kinetic energy , kinetics , molecule , fluorescence , chemistry , biological system , extraction (chemistry) , chemical physics , physics , stereochemistry , chromatography , biology , organic chemistry , quantum mechanics
We discuss two possible approaches for extracting kinetic information from single‐molecule experiments. The first approach is based on computing correlation functions from measured fluorescence signals, and the second on studying the statistics of on and off times of the same fluorescence signal. We show that in both cases it is possible to extract kinetic information about the nature of intramolecular fluctuations of the single molecule. We show that for single‐molecule kinetics the intramolecular fluctuations produce stochastic memory effects which lead to new dynamic features that do not exist in traditional chemical kinetics. In particular, we investigate a new type of chemical oscillations in correlation functions observed experimentally by Edman and Rigler (Proc. Natl. Acad. Sci. USA 2000 , 97, 8266).