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Functional Microdomains of Glycolipids with Partially Fluorinated Membrane Anchors: Impact on Cell Adhesion
Author(s) -
Gege Christian,
Schneider Matthias F.,
Schumacher Gabriele,
Limozin Laurent,
Rothe Ulrich,
Bendas Gerd,
Tanaka Motomu,
Schmidt Richard R.
Publication year - 2004
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200300947
Subject(s) - alkyl , lipid microdomain , chemistry , monolayer , membrane , lipid bilayer , glycolipid , vesicle , adhesion , glycan , chinese hamster ovary cell , biophysics , bilayer , cell adhesion , biochemistry , organic chemistry , receptor , glycoprotein , biology
Functional microdomains of glycolipids were designed by mixing neoglycolipids with partially fluorinated alkyl (F‐alkyl) chains and matrix lipids with alkyl chains. Fluorescence images of the mixed lipid monolayers at the air–water interface demonstrated that it is possible to control both size and distribution of the microdomains by means of the strong demixing of alkyl and F‐alkyl membrane anchors, while the carbohydrate head groups seemed to play a rather minor role. These microdomains in monolayers could be transferred onto hydrophobized substrates and subjected to experiments in a dynamic flow chamber. The results obtained here clearly indicated that the dynamic adhesion of Chinese hamster ovarial cells expressing E‐selectin (CHO‐E cells) on a lipid monolayer containing microdomains of sialyl Lewis X (sLe X ) can be both enhanced and reduced by controlled demixing of ligands and matrices. Moreover, the same clusters of sLe X could also be formed in giant lipid vesicles, which can be used as a model cell that locally expresses biospecific functions.