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Maximum‐Likelihood Approach to Single‐Molecule Polarization Modulation Analysis
Author(s) -
Osborn Kenneth D.,
Singh Manoj K.,
Bieber Urbauer Ramona J.,
Johnson Carey K.
Publication year - 2003
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200300677
Subject(s) - polarization (electrochemistry) , calmodulin , molecule , chemistry , modulation (music) , fluorescence , fluorescence anisotropy , biophysics , molecular physics , membrane , chemical physics , analytical chemistry (journal) , calcium , physics , optics , chromatography , biochemistry , acoustics , biology , organic chemistry
Mobile or not mobile? Polarization modulation has been used to probe single‐molecule dynamic heterogeneity of dye molecules that are either surface‐bound or incorporated in the biological system calmodulin–plasma membrane calcium ATPase. A maximum‐likelihood approach was applied to determine modulation depth and phase shift of the fluorescence trajectories (see picture), which give a measure of the orientational mobility of the molecules.