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Mechanism of Lck Recruitment to the T‐Cell Receptor Cluster as Studied by Single‐Molecule‐Fluorescence Video Imaging
Author(s) -
Ike Hiroshi,
Kosugi Atsushi,
Kato Akiko,
Iino Ryota,
Hirano Hidemi,
Fujiwara Takahiro,
Ritchie Ken,
Kusumi Akihiro
Publication year - 2003
Publication title -
chemphyschem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.016
H-Index - 140
eISSN - 1439-7641
pISSN - 1439-4235
DOI - 10.1002/cphc.200300670
Subject(s) - jurkat cells , t cell receptor , green fluorescent protein , chemistry , biophysics , fluorescence , molecule , cluster (spacecraft) , small molecule , microbiology and biotechnology , biology , t cell , biochemistry , computer network , physics , genetics , gene , computer science , optics , immune system , organic chemistry
Molecular movies: The Src‐family kinase Lck is recruited to the site of Tcell receptor (TCR) clustering at very early stages after TCR engagement. The authors have investigated the mechanism of Lck recruitment to the TCR cluster by monitoring the movement of single molecules of Lck and its N ‐terminal ten amino acid sequence (N10; for experimental procedure see picture), both fused with green fluorescent protein (GFP) and expressed in the Jurkat Tcell line. Single‐molecule observations revealed that neither Lck‐ nor N10‐GFP underwent directed movement toward the cluster, but they both underwent apparent short‐term simple Brownian diffusion.