Open AccessGenome Editing with CRISPR‐Cas: An Overview
Author(s) -
Robb G. Brett
Publication year - 2019
Publication title -
current protocols essential laboratory techniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.747
H-Index - 10
ISSN - 1948-3430
DOI - 10.1002/cpet.36
Subject(s) - crispr , genome editing , palindrome , genome , computational biology , biology , genetics , guide rna , dna , gene
The understanding and application of clustered regularly interspaced short palindromic repeat (CRISPR) systems and CRISPR‐associated (Cas) nucleases have helped genome editing become a standard laboratory technique. CRISPR‐Cas nucleases are RNA‐programmed DNA‐cutting enzymes that facilitate the introduction of intentional sequence changes into the genomes of experimental cells and organisms. This overview provides a background for genome editing and CRISPR‐Cas nucleases, as well as an overview of the technology used to assess the outcomes of genome editing. © 2019 The Authors.