Open AccessVisualizing RNA Cytidine Acetyltransferase Activity by Northern Blotting
Author(s) -
Bryson Keri M.,
ThalallaGamage Supuni,
Meier Jordan L.
Publication year - 2020
Publication title -
current protocols in chemical biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.503
H-Index - 14
ISSN - 2160-4762
DOI - 10.1002/cpch.89
Subject(s) - blot , cytidine , acetyltransferases , acetyltransferase , histone acetyltransferases , northern blot , rna , cytidine deaminase , microbiology and biotechnology , biology , biochemistry , chemistry , enzyme , gene , gene expression , acetylation
Cytidine acetyltransferases are an emerging class of nucleic‐acid‐modifying enzymes responsible for the establishment of N 4 ‐acetylcytidine (ac4C) in RNA. In contrast to histone acetyltransferases, whose activity is commonly studied by western blotting, relatively few methods exist for quickly assessing the activity of cytidine acetyltransferases from a biological sample of interest or the distribution of ac4C across different RNA species. In this protocol, we describe a method for analysis of cellular cytidine acetyltransferase activity using dot‐ and immuno‐northern‐blotting‐based detection. © 2020 Wiley Periodicals LLC. Basic Protocol 1 : Detection of N 4 ‐Acetylcytidine in RNA by dot blotting Basic Protocol 2 : Visualizing N 4 ‐Acetylcytidine Distribution in RNA by northern blotting