Identification of Phosphorylated Proteins on a Global Scale | Zendy

Iliuk Anton | Zendy

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Identification of Phosphorylated Proteins on a Global Scale

Author(s) -

Iliuk Anton

Publication year - 2018

Publication title -

current protocols in chemical biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.503

H-Index - 14

ISSN - 2160-4762

DOI - 10.1002/cpch.48

Subject(s) - computational biology , identification (biology) , phosphorylation , tandem mass spectrometry , chemistry , mass spectrometry , protein phosphorylation , posttranslational modification , liquid chromatography–mass spectrometry , proteomics , chromatography , sample preparation , biology , biochemistry , enzyme , protein kinase a , botany , gene

Liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS) has enabled researchers to analyze complex biological samples with unprecedented depth. It facilitates the identification and quantification of modifications within thousands of proteins in a single large‐scale proteomic experiment. Analysis of phosphorylation, one of the most common and important post‐translational modifications, has particularly benefited from such progress in the field. Here, detailed protocols are provided for a few well‐regarded, common sample preparation methods for an effective phosphoproteomic experiment. © 2018 by John Wiley & Sons, Inc.

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