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Construction of FRET‐Based Off‐On Fluorescent Nanoprobes for Sensitive Detection of Intracellular Singlet Oxygen
Author(s) -
Ping Jiantao,
Qin Jinglei,
Zhou Chao,
Guo Lanying,
Liu Bei,
Chen Zhihua,
Geng Zhaoxin,
Peng Hongshang
Publication year - 2020
Publication title -
chemnanomat
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 32
ISSN - 2199-692X
DOI - 10.1002/cnma.201900571
Subject(s) - nanoprobe , fluorescence , förster resonance energy transfer , singlet oxygen , photochemistry , biocompatibility , two photon excitation microscopy , materials science , detection limit , conjugated system , nanotechnology , chemistry , nanoparticle , polymer , oxygen , optics , chromatography , physics , organic chemistry , metallurgy , composite material
Singlet oxygen ( 1 O 2 ) detection remains an active area of research in the field of biological and biomedical science. In this work, we reported a novel Off‐On type FRET‐based fluorescent 1 O 2 nanoprobe comprising the energy‐donating conjugated polymer, poly(9,9‐dioctylfluorenyl‐2,7‐diyl) (PFO), and the energy‐accepting 1 O 2 indicator, 3‐diphenylisobenzofuran (DPBF). Prior to the reaction with 1 O 2 , the nanoprobe was in Off‐state because fluorescence of PFO ( λ em =441 nm) was completely quenched by loaded DPBF; in the presence of 1 O 2 , it switched to On‐state due to the depletion of DPBF. The as‐prepared 1 O 2 nanoprobe had a particle size of ∼59 nm, good biocompatibility, high specificity and sensitivity with a limit of detection of 353 nM. Importantly, the fluorescent nanoprobes were highly sensitive to 1 O 2 under two‐photon excitation ( λ ex =800 nm). Using a multiphoton confocal microscope, generation of intracellular 1 O 2 during photodynamic therapy was sensitively detected by recording fluorescence intensity of the nanoprobes.