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Study of Epitope Imprinting for Small Templates: Preparation of NanoMIPs for Ochratoxin A
Author(s) -
LópezPuertollano Daniel,
Cowen Todd,
GarcíaCruz Álvaro,
Piletska Elena,
AbadSomovilla Antonio,
AbadFuentes Antonio,
Piletsky Sergey
Publication year - 2019
Publication title -
chemnanomat
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 32
ISSN - 2199-692X
DOI - 10.1002/cnma.201900050
Subject(s) - ochratoxin a , chemistry , mycotoxin , molecularly imprinted polymer , hapten , ochratoxin , chromatography , surface plasmon resonance , molecular imprinting , nanotechnology , nanoparticle , food science , materials science , organic chemistry , antibody , biology , immunology , selectivity , catalysis
Ochratoxin A (OTA) is a carcinogenic mycotoxin produced by Aspergillus and Penicillium, which contaminates cereals like corn and wheat, in addition to beverages such as coffee, wine and beer. Chromatography is the preferred analytical method for OTA determination, but unfortunately this method is still elaborate, expensive and tedious. Alternative mycotoxin detection techniques involve assays and sensors based on molecularly imprinted polymers nanoparticles (nanoMIPs) as synthetic polymeric receptors mimicking antibodies. Herein we designed nanoMIPs for specific recognition of OTA using computational screening and docking of different acryl functional monomers. Subsequently, different nanoMIPs were prepared by solid phase synthesis using two OTA haptens, OTA e‐ MIPs without carboxylic group and OTA b‐ MIPs bearing the carboxylic group. NanoMIPs affinity towards OTA was analyzed by surface plasmon resonance, the results displayed dissociation constants for OTA b‐ MIPs at 0.4 μM and for OTA e‐ MIPs at 5.1 μM. Therefore, the results demonstrate that nanoMIPs recognition depends on the immobilization position of the OTA hapten as observed using antibodies.