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Design of Colloidally Stable and Non‐Toxic Petox‐Based Polymersomes for Cargo Molecule Encapsulation
Author(s) -
Oz Umut Can,
Kucukturkmen Berrin,
Ozkose Umut Ugur,
Gulyuz Sevgi,
Bolat Zeynep Busra,
Telci Dilek,
Sahin Fikrettin,
Yilmaz Ozgur,
Bozkir Asuman
Publication year - 2019
Publication title -
chemnanomat
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 32
ISSN - 2199-692X
DOI - 10.1002/cnma.201800606
Subject(s) - polymersome , amphiphile , copolymer , polymer , materials science , polymer chemistry , chemistry , viability assay , chemical engineering , nanotechnology , cell , organic chemistry , biochemistry , engineering
Polymersomes are vesicular constructs formed by the self‐assembly of polymer amphiphiles. Here we report the fabrication of PEtOx‐ b ‐PLA polymersomes at physiological pH/salt concentration and the determination of a f PEtOx range, which is one of the most recognized factors that governs the self‐assembly of amphiphilic copolymers, for the first time. The findings indicate that PEtOx‐ b ‐PLA copolymers, containing PEtOx fractions of varied lengths, self‐assemble to form polymersomes in the range of 98.35 to 119.50 nm. The encapsulation potential of PEtOx‐ b ‐PLA polymersomes was demonstrated by loading of docetaxel (hydrophobic) with 17.90% encapsulation efficiency and by loading of pDNA (hydrophilic) with 65.03% encapsulation efficiency. In addition, size distribution profiles showed that polymersomes are colloidally stable for 24 h at 37 °C and for 3 months at +4 °C. The cell viability results showed that PEtOx‐ b ‐PLA copolymer and polymersomes are not cytotoxic to HUVEC, HEK293 and hMSC cell lines.