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Protein Corona Formation on Magnetic Nanoparticles Conjugated with Luminescent Europium Complexes
Author(s) -
Khan Latif U.,
Petry Romana,
Paula Amauri J.,
Knobel Marcelo,
Martinez Diego Stéfani T.
Publication year - 2018
Publication title -
chemnanomat
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 32
ISSN - 2199-692X
DOI - 10.1002/cnma.201800358
Subject(s) - europium , nanoparticle , luminescence , bifunctional , context (archaeology) , chemistry , protein adsorption , corona (planetary geology) , adsorption , analytical chemistry (journal) , materials science , nanotechnology , organic chemistry , paleontology , physics , optoelectronics , astrobiology , venus , biology , catalysis
The evaluation of the nanoparticles interactions with blood plasma enables a first and important insight on the organization of the adsorbed protein layer. In this context, we studied the formation of protein corona on magneto‐luminescent Fe 3 O 4 @calix‐Eu(TTA) nanoparticles in human plasma. The difference in the surface chemistry of F 3 O 4 functionalized with calixarene (+30 mV ζ‐potential) and europium (III) thenoyltrifluoroacetonate (Eu 3+ ‐TTA) complex (+7.4 mV ζ‐potential) affected the colloidal stability and hard corona composition of the nanoparticles, which were monitored by SDS‐PAGE gel electrophoresis, differential centrifugal sedimentation analyses and luminescence spectroscopy. Strikingly, after conjugation with Eu 3+ TTA complex, the magnetic nanoparticles show lower adsorption affinity to proteins at higher blood plasma concentration. Moreover, the Eu 3+ compound is featured with the narrow emission lines of 5 D 0 → 7 F J (J=0–4) transitions, imparting additional characteristics to bifunctional nanoparticles that were used as probes to evaluate the protein corona formation.