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Mineralized Peptide Nanofiber Gels for Enhanced Osteogenic Differentiation
Author(s) -
Eren E. Deniz,
Tansik Gulistan,
Tekinay Ayse B.,
Guler Mustafa O.
Publication year - 2018
Publication title -
chemnanomat
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 32
ISSN - 2199-692X
DOI - 10.1002/cnma.201700354
Subject(s) - mineralization (soil science) , biomineralization , chemistry , peptide , nanofiber , nucleation , biophysics , mineralized tissues , calcium , transmission electron microscopy , phosphate , chemical engineering , biochemistry , nanotechnology , materials science , organic chemistry , biology , dentin , nitrogen , engineering , composite material
Abstract Mineral deposition is observed in both bacterial and eukaryotic organisms through a broad range of mechanisms. Both organic and inorganic components play crucial roles in the formation of mineralized tissues, and acidic proteins are particularly important in this context owing to their ability to stimulate nucleation of minerals. Here, we present negatively‐charged self‐assembling peptide amphiphile molecules as a template to nucleate calcium phosphate mineralization in a bioactive scaffold environment. Acidic peptide molecules were shown to induce formation of hydroxyapatite like calcium phosphate mineralization, which was characterized by scanning electron microscopy, transmission electron microscopy, thermogravimetric analysis, X‐ray diffractometry, oscillatory rheology and atomic force microscopy. The osteoblast‐like cells were found to reveal enhanced osteogenic differentiation on pre‐mineralized peptide nanofiber networks, suggesting that mineral deposition can be used as a means of enhancing the bioactivity of peptide‐based scaffold systems.