Premium
Dual‐Fluorescent Nanoscale Coordination Polymers via a Mixed‐Ligand Synthetic Strategy and Their Use for Multichannel Imaging
Author(s) -
Nador Fabiana,
Wnuk Karolina,
GarcíaPardo Javier,
Lorenzo Julia,
Solorzano Rubén,
RuizMolina Daniel,
Novio Fernando
Publication year - 2018
Publication title -
chemnanomat
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 32
ISSN - 2199-692X
DOI - 10.1002/cnma.201700311
Subject(s) - bifunctional , chemistry , fluorescence , ligand (biochemistry) , combinatorial chemistry , nanoparticle , hydrazide , covalent bond , imidazole , catechol , photochemistry , polymer chemistry , organic chemistry , nanotechnology , materials science , quantum mechanics , biochemistry , physics , receptor , catalysis
Two rationally designed strategies for covalent bonding of fluorescent dyes in coordination polymer nanoparticles aiming to achieve bifunctional fluorescent nanostructures have been developed. The first strategy was based on the synthesis of the coordination polymers structured as nanoparticles by coordination of Co II ions to two different catechol ligands containing free functional chemical groups (dopamine and 3,4‐dihydroxybenzaldehyde), and a bis(imidazole)‐based ligand (1,4‐bis(imidazole‐1‐ylmethyl)benzene, bix). Subsequently, different dyes, namely fluorescein isothiocyanate (FITC), 1‐pyrenebutanoic acid hydrazide (PBH) or Alexa Fluor® 568 (A568), could be sequentially attached to the surface of the nanoparticles. The second strategy was focused on the prefunctionalization of catechol ligands with the corresponding dyes and, afterwards, the coordination with the metal ions in presence of bix. In vitro studies demonstrated the internalization of the bifunctional nanoparticles and the persistence of the fluorescent properties after cell uptake without dye leaching.