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Replication of Polymer‐Based Peptide Microarrays by Multi‐Step Transfer
Author(s) -
Striffler Jakob,
Mattes Daniela S.,
Schillo Sebastian,
Münster Bastian,
Palermo Andrea,
Ridder Barbara,
Welle Alexander,
Trouillet Vanessa,
Stadler Volker,
Markovic Goran,
Proll Günther,
Bräse Stefan,
Loeffler Felix F.,
NesterovMüller Alexander,
Breitling Frank
Publication year - 2016
Publication title -
chemnanomat
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 32
ISSN - 2199-692X
DOI - 10.1002/cnma.201600194
Subject(s) - peptide , streptavidin , dna microarray , azide , chemistry , combinatorial chemistry , replication (statistics) , nanotechnology , computational biology , materials science , biology , biochemistry , biotin , gene , organic chemistry , gene expression , virology
Peptide microarrays serve as a high‐throughput tool for the readout of protein–peptide interactions. We present a multi‐step purification of high‐complexity peptide microarrays by transfer to specialized surfaces. Only correctly synthesized peptides are transferred from the synthesis surface to various substrates where they can be immobilized by a variety of different anchor molecules. Here, we demonstrate the transfer to rigid streptavidin, epoxide, gold, and azide surfaces. The reported method reduces the cost of purified peptide array production through the reproduction of one array several times.