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Nanostructure‐Dependent Ratiometric NIR Fluorescence Enabled by Ordered Dye Aggregation
Author(s) -
Charron Danielle M.,
Chen Juan,
Zheng Gang
Publication year - 2016
Publication title -
chemnanomat
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 32
ISSN - 2199-692X
DOI - 10.1002/cnma.201600038
Subject(s) - nanocarriers , fluorescence , nanostructure , nanotechnology , biophysics , chemistry , fluorescence lifetime imaging microscopy , materials science , nanoparticle , biology , physics , quantum mechanics
Nanocarriers incorporating therapeutic and imaging agents within a single nanostructure are emerging tools for drug development and treatment planning. Additional information can be provided using activatable fluorescence that dynamically reports nanocarrier disruption and drug release. Dual‐wavelength activation encodes a unique fluorescence signal for each nanocarrier state, enabling use of ratiometric imaging to measure the proportion of intact and disrupted nanocarriers within tissues. Here we investigate dual‐wavelength activation using a single dye whose optical properties are intricately linked to the nanocarrier structure. Natural bacteriochlorophyll incorporated within a compact lipoprotein nanocarrier forms ordered dye aggregates with red‐shifted fluorescence emission from 765 nm to 825 nm. We demonstrate that bacteriochlorophyll‐ordered aggregation is a feasible strategy to distinguish intact and disrupted theranostic nanocarriers by ratiometric fluorescence imaging.