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Monitoring the Intracellular Distribution and ROS Scavenging Potential of Carbon Dot–Cerium Oxide Nanocomposites in Fibroblast Cells
Author(s) -
Sachdev Abhay,
Gopinath P.
Publication year - 2016
Publication title -
chemnanomat
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.947
H-Index - 32
ISSN - 2199-692X
DOI - 10.1002/cnma.201500224
Subject(s) - cerium oxide , nanocomposite , reactive oxygen species , flow cytometry , oxidative stress , fibroblast , intracellular , dichlorofluorescein , fluorescence microscope , materials science , biophysics , chemistry , nanotechnology , oxide , fluorescence , microbiology and biotechnology , biochemistry , in vitro , biology , metallurgy , physics , quantum mechanics
In the present work, cerium oxide (CeO 2 )‐based nanocomposites integrating fluorescent carbon dots (CDs) were synthesized by a hydrothermal method. The formation and cellular uptake of these nanocomposites was studied by various analytical techniques. The therapeutic and imaging aspects of CDs‐CeO 2 nanocomposites were validated using NIH3T3 fibroblast cells as a model system. The antioxidant potency of CDs‐CeO 2 nanocomposites was also evaluated in cells treated with H 2 O 2 , a major reactive oxygen species (ROS) species, using a 2′,7′‐dichlorofluorescin diacetate (DCFH‐DA) assay. Tracking the intracellular fluorescence of CDs‐CeO 2 nanocomposites by microscopy and flow cytometry made it possible to examine the capabilities of CeO 2 for abatement of H 2 O 2 ‐mediated oxidative stress. Taken together, the present study motivates the use of therapeutic nanocomposites whilst monitoring their uptake, which holds clinical relevance for ROS‐related diseases.