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Central nervous system neurons infected by pseudorabies virus injected into the rat urinary bladder following unilateral transection of the pelvic nerve
Author(s) -
Nadelhaft Irving,
Vera Pedro L.
Publication year - 1995
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903590307
Subject(s) - spinal cord , anatomy , biology , pseudorabies , brainstem , central nervous system , urinary bladder , hypogastric nerve , locus coeruleus , rhizotomy , dorsum , medicine , stimulation , virus , neuroscience , virology
Following the transection of one pelvic nerve and both hypogastric nerves, the urinary bladder of male Sprague‐Dawley rats was injected with pseudorabies virus (PRV; Bartha strain). The central stump of the transected pelvic nerve was labelled with fast blue (FB), and rats were maintained for 2, 2. 5, and 3 days following viral infection. Tissue was processed with antisera against PRV and choline acetyltransferase (CAT). In the L6‐S1 spinal cord, neurons in the ipsilateral intermediolateral area (IML) were labelled after 2 days. After 2. 5 days, labelled neurons were also found in the dorsal gray commissure (DGC), the ipsilateral superficial dorsal horn, and the contralateral IML area. After 3 days, many labelled neurons appeared in the superficiai dorsal horns and, bilaterally, in the L6‐S1 dorsal root ganglia. In both IMLs, two groups of PRV‐labelled neurons were found: (1) CAT‐positive preganglionic cells and (2) smaller, CAT‐negative cells located slightly dorsal to the preganglionic neurons. No other doubly stained neurons were found in the spinal cord. Contralateral DRG neurons stained for either PRV or FB or both. Ipsilateral DRG neurons stained only for PRV. PRV‐immunoreactive (IR) neurons appeared in the brainstem only after 3 days. These were located primarily in the pontine micturition centers (equal numbers), the ventral locus coeruleus, and the raphe and lateral reticular areas. © 1995 Wiley‐Liss, Inc.