Premium
Expression of the p150 Glued component of the dynactin complex in developing and adult rat brain
Author(s) -
Melloni Richard H.,
Tokito Mariko K.,
Holzbaur Erika L. F.
Publication year - 1995
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903570103
Subject(s) - biology , in situ hybridization , neuropil , synaptogenesis , neuroscience , granule cell , cerebellum , axoplasmic transport , microbiology and biotechnology , central nervous system , anatomy , dentate gyrus , messenger rna , genetics , gene
Abstract p150 Glued is a component of the dynactin (Glued) complex that has been shown in vitro to be a required activator of cytoplasmic dyneinmediated transport of vesicles along microtubules and, thus, may be an essential component of retrograde axonal transport. In vivo, a dominant mutation in the Drosophila homologue of p150 Glued induces aberrant neuronal development when heterozygous and is lethal when homozygous. In order to characterize the role of the dynactin complex in the development and function of vertebrate neurons, the distribution of the p150 Glued message was examined via in situ hybridization to serial sections of adult rat brain and to a developmental series of sections. In the adult rat brain, the most intense hybridization observed with the p150 Glued probe was in the pyramidal cells of the hippocampus proper, the dentate granule neurons, the cingulate and piriform cortices, the ventromedial hypothalamus, and the granular cell layer of the cerebellum. White‐matter fiber tracts and the neuropil were generally devoid of signal. The data indicate that the mRNA encoding p150 Glued is highly enriched in the cell bodies of neurons within the central nervous system. In developing rat, p150 Glued is expressed at very high levels in neural tissue from the earliest time points assayed. Particularly intense hybridization was observed in the multiple layers of the retina, which is consistent with the phenotype of the Drosophila mutation. Therefore, the distributions observed via in situ hybridization are consistent with an essential role for p150 Glued in retrograde axonal transport. © 1995 Wiley‐Liss, Inc.