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Ultrastructural localization of SNAP‐25 within the rat spinal cord and peripheral nervous system
Author(s) -
Duc Christophe,
Catsicas Stefan
Publication year - 1995
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903560111
Subject(s) - axolemma , biology , axoplasm , axon , immunocytochemistry , synaptic vesicle , golgi apparatus , compartment (ship) , ultrastructure , central nervous system , neuroscience , spinal cord , vesicle , peripheral nervous system , axon terminal , axoplasmic transport , compartmentalization (fire protection) , microbiology and biotechnology , endoplasmic reticulum , nervous system , anatomy , membrane , biochemistry , myelin , endocrinology , oceanography , geology , enzyme
Abstract Synaptosomal associated protein of 25 kDa (SNAP‐25) has been implicated in the membrane fusion machinery of neurotransmitter release and axonal growth. Using immunocytochemistry, we have analyzed the distribution and ultrastructural localization of SNAP‐25 in selected areas of the central and peripheral nervous systems of adult rats. We show that the protein is specifically expressed in the trans face of the Golgi apparatus and in the axonal compartment. In axons and nerve endings, SNAP‐25 is localized to discrete areas of the membranes of most organelles such as the axoplasmic reticulum, the axolemma, the outer membrane of mitochondria and synaptic vesicles. This wide distribution of SNAP‐25 suggests that the protein is involved in the fusion of membranes in the whole axonal compartment of neurons. © 1995 Wiley‐Liss, Inc.