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Distribution of metabotropic glutamate receptor mGluR5 immunoreactivity in rat brain
Author(s) -
Romano Carmelo,
Sesma Michael A.,
McDonald Colin T.,
O'malley Karen,
van den Pol Anthony N.,
Olney John W.
Publication year - 1995
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903550310
Subject(s) - metabotropic glutamate receptor 5 , biology , metabotropic glutamate receptor 4 , metabotropic glutamate receptor 3 , metabotropic glutamate receptor 8 , metabotropic glutamate receptor , metabotropic glutamate receptor 6 , metabotropic glutamate receptor 1 , metabotropic glutamate receptor 7 , neuroscience , metabotropic glutamate receptor 2 , glutamate receptor , receptor , biochemistry
The receptor mGluR5 is a metabotropic glutamate receptor with messenger RNA abundantly present throughout cortex, hippocampus, and caudate/putamen that is also coupled to phosphatidyl inositide hydrolysis and calcium mobilization. In this study, the distribution of mGluR5 was examined in rat brain by immunocytochemistry. The antibody utilized is highly specific and does not cross react with the most closely related other metabotropic glutamate receptor, as determined by Western blot analysis of nonneuronal cells transfected with metabotropic receptor coding sequences. The receptor mGluR5 is widely expressed with the highest density in olfactory bulb, caudate/putamen, lateral septum, cortex, and hippocampus, as confirmed with both immunocytochemistry and Western blot analysis. Electron microscopic studies in hippocampus and cortex indicate that the labeling is mostly on membranes of dendritic spines and shafts. Light and electron microscopic evidence indicates that some mGluR5 immunoreactivity is located in presynaptic axon terminals, suggesting that mGluR5 may function as a presynaptic receptor.