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Expression of four immunoglobulin superfamily adhesion molecules (L1, Nr‐CAM/Bravo, neurofascin/ABGP, and N‐CAM)in the developing mouse spinal cord
Author(s) -
Moscoso Lisa M.,
Sanes Joshua R.
Publication year - 1995
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903520302
Subject(s) - biology , spinal cord , in situ hybridization , neural cell adhesion molecule , microbiology and biotechnology , cell adhesion molecule , embryonic stem cell , immunoglobulin superfamily , gdf7 , immunohistochemistry , messenger rna , cell adhesion , anatomy , gene , neuroscience , cell , immunology , genetics
To identify cell adhesion molecules (CAMs) expressed by mammalian motoneurons, we applied the polymerase chain reaction to a murine motor neuron‐like cell line, NSC‐34. Using primers derived from a group of Ll‐related CAMs, we cloned two alternatively spliced forms of mouse L1, which differ by a 12‐base‐pair insert, plus putative murine orthologs of the chicken cell adhesion molecules Nr‐CAM/Bravo and neurofascin. All four mRNAs are expressed in NSC‐34 cells, but only neurofascin and the insert‐minus form of Ll are expressed in its neuroblastoma parent, N18TG2. Analysis of RNA in neonatal tissues reveals expression largely restricted to the brain and spinal cord. In situ hybridization histochemistry of spinal cord shows that motoneurons express L1, Nr‐CAM, and neurofascin as well as N‐CAM. Ll and N‐CAM RNAs are detected throughout the period studied (from embryonic day,[E]11 to postnatal day [P]28), whereas Nr‐CAM is expressed only at early ages (