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Characterization of trout galanin and its distribution in trout brain and pituitary
Author(s) -
Anglade Isabelle,
Wang Yunxia,
Jensen Jorgen,
Tramu Gerard,
Kah Olivier,
Conlon J. Michael
Publication year - 1994
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903500105
Subject(s) - galanin , medicine , endocrinology , hypothalamus , diencephalon , cerebrum , biology , trout , median eminence , pars intermedia , pituitary gland , neuropeptide , midbrain , posterior commissure , central nervous system , hormone , nucleus , receptor , neuroscience , fishery , fish <actinopterygii>
Galanin was purified from an extract of the stomach of the rainbow trout, Oncorhynchus mykiss , and its primary structure was established as Gly‐Trp‐Thr‐Leu‐Asn‐Ser‐Ala‐Gly‐Tyr‐Leu 10 ‐Leu‐Gly‐Pro‐His‐Gly‐Ile‐Asp‐Gly‐His‐Arg 20 ‐Thr‐Leu‐Ser‐Asp‐Lys‐His‐Gly‐Leu‐Ala. Trout galanin shows six amino acid substitutions compared with pig galanin, but the N‐terminal region (residues 1–14) has been fully conserved. The distribution of galanin‐immunoreactive (GAL‐IR) structures in the trout brain and pituitary was studied via immunohistochemistry. GAL‐IR cell bodies were observed only in the caudal telencephalon, the preoptic region, and the mediobasal hypothalamus. GAL‐IR fibers, however, are widely distributed throughout the brain, with a much lower density in the midbrain and posterior brain than in the tel‐ and diencephalon. Particularly dense innervation of the mediobasal hypothalamus, the ventral and supracommissuralis parts of the caudal telencephalon, and the region above and below the anterior commissure was observed. A heavy innervation of the pituitary was consistently detected. GAL‐IR fibers were present in neurohypophyseal digitations of both the anterior and intermediate lobes with highest density in the region of the proximal pars distalis, where growth hormone and gonadotropic cells are located. Fibers were also seen in digitations of the rostral pars distalis, in particular between the prolactin follicles. The distribution of GAL‐IR neurons in the central nervous system and pituitary of the trout suggests that the peptide may exercise an important role in the regulation of neuroendocrine functions, particularly those related to reproduction. © 1994 Wiley‐Liss, Inc.