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Imaging of individual normal and regenerating optic fibers in the brain of living adult goldfish
Author(s) -
Danks Anne M.,
Kim Patricia,
Wang Ziren,
Meyer Ronald L.
Publication year - 1994
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903450207
Subject(s) - tectum , biology , optic nerve , optic tectum , retina , anatomy , axon , retinal , fish <actinopterygii> , optic cup (embryology) , axoplasmic transport , neuroscience , midbrain , central nervous system , biochemistry , fishery , gene , eye development , phenotype
Retinal arbors in the tectum of living adult goldfish were imaged to determine whether the structural remodelling and refinement that occurs during development continues in adulthood. Individual optic fibers were labelled by making small injections of the lipophilic fluorescent dye DiI into ventral retina and viewing the exposed tectum through a fluorescence microscope equipped with a cooled CCD camera. Arbors were imaged in the living fish every 30–60 minutes for up to 7 hours. Normal adult goldfish showed no evidence of arbor remodelling during this period, though dynamic movements of varicosities present along axon segments were observed. For comparison, regenerating optic fibers were similarly imaged in fish that had undergone optic nerve crush 2–6 weeks previously. In these fish, dynamic structural changes were seen, including branch remodelling, extension and retraction of growth cones, and movement of varicosities. © 1994 Wiley‐Liss, Inc.