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Immunolocalization of aromatic L‐amino acid decarboxylase in goldfish ( Carassius auratus ) brain
Author(s) -
Beltramo M.,
Krieger M.,
Tillet Y.,
Thibault J.,
Calas A.,
Mazzi V.,
Franzoni M. F.
Publication year - 1994
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903430203
Subject(s) - aromatic l amino acid decarboxylase , tyrosine hydroxylase , aromatic amino acids , serotonin , monoamine neurotransmitter , biology , tyrosine , amino acid , biochemistry , tryptophan hydroxylase , enzyme , glutamate decarboxylase , tyrosine 3 monooxygenase , medicine , endocrinology , receptor , serotonergic
The distribution of monoamines (catecholamines and serotonin) in fishes has been previously studied by immunohistochemistry of both the monoamines themselves and their biosynthetic enzymes. But the distribution of neuron containing aromatic L‐amino acid decarboxylase, and enzyme involved in the biosynthesis of both catecholamines and serotonin, has up to now not been investigated. In order to improve knowledge about the localization of aromatic L‐amino acid decarboxylase, neurons containing this enzyme were mapped immunohistochemically in the goldfish brain. Furthermore, neurons bearing aromatic L‐amino acid decarboxylase immunoreactivity have been compared with those containing tyrosine hydroxylase and serotonin immunoreactivities. Our results show that distribution of aromatic L‐amino acid decarboxylase immunoreactivity generally coincides with that of tyrosine hydroxylase and serotonin. Nevertheless, the presence of nine D cell groups (containing aromatic L‐amino acid decarboxylase but lacking both catecholamines and serotonin) and six groups of neurons which are aromatic L‐amino acid decarboxylase‐immunoreactive but contain tyrosine hydroxylase, and might produce L‐DOPA, have been revealed. The occurrence of both D cell groups and presumptive L‐DOPA neurons in goldfish brain is discussed in relation to similar findings in fish and mammalian brain. © 1994 Wiley‐Liss, Inc.