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Ultrastructural evidence for intra‐ and extranuclear projections of GABAergic neurons of the suprachiasmatic nucleus
Author(s) -
Buijs Ruud M.,
Hou YangXun,
Shinn Susan,
Renaud Leo P.
Publication year - 1994
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903400308
Subject(s) - suprachiasmatic nucleus , gabaergic , biology , immunocytochemistry , nucleus , hypothalamus , neuroscience , immunogold labelling , circadian rhythm , neurotransmitter , ultrastructure , anterograde tracing , inhibitory postsynaptic potential , central nervous system , endocrinology , anatomy
GABAergic projections of the suprachiasmatic nucleus (SCN) were demonstrated in a double‐labelling ultrastructural study which visualised the efferents of the SCN by PHA‐L tracing, diaminobenzidine (DAB) immunocytochemistry, and GABA with immunogold postembedding staining. The results show a strong contralateral projections of the SCN that is partly GABA‐containing. In addition, ipsilateral SCN projections to the dorsomedial hypothalamus and periventricular part of the paraventricular nucleus and subparaventricular nucleus were shown to contain GABA. The present results indicate that the SCN may utilize this inhibitory neurotransmitter to regulate and organize its own circadian rhythm as well as using GABA to transmit its diurnal information to other regions of the brain. © Wiley‐Liss, Inc.