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Complex expression of keratins in goldfish optic nerve
Author(s) -
Druger Robert K.,
Glasgow Eric,
Fuchs Chana,
Levine Edward M.,
Matthews James P.,
Park ChongYon,
Schechter Nisson
Publication year - 1994
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903400211
Subject(s) - keratin , biology , optic nerve , keratin 6a , in situ hybridization , messenger rna , keratin 8 , intermediate filament , microbiology and biotechnology , keratin 7 , gene expression , complementary dna , anatomy , cytoskeleton , gene , genetics , cell , immunohistochemistry , cytokeratin , immunology
Abstract Keratins are the predominant intermediate filament proteins in the nonneuronal cells of the goldfish optic nerve. At least three different keratin pairs are expressed in this tissue, indicating an unexpected complexity. Expression of the type II keratin ON 3 in goldfish opticnerve astrocytes predicts the expression of a type I keratin partner. Here we report the cDNA sequence and predicted amino acid sequence of two type I keratins from the goldfish optic nerve, designated GK48 and GK49. The GK48 protein is the goldfish equivalent of mammalian keratin 18 (K18) and is the most likely type I keratin partner to the ON 3 protein. The GK49 protein is similar to the GK50 protein, a type I keratin characterized previously from the goldfish optic nerve. The GK48 and ON 3 mRNAs are expressed in a variety of goldfish tissues, whereas the expression of GK49 mRNA has a more limited expression. In addition, in situ hybridization experiments show that the expression of the GK48 and ON 3 mRNAs are evenly distributed throughout the optic nerve, while the GK49 mRNA is expressed along longitudinal lines. These results show that there is a diversity of keratin expression within different cell types in the goldfish optic nerve. © Wiley‐Liss, Inc.