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Cholinergic innervation of olfactory glomeruli in the rat: An ultrastructural immunocytochemical study
Author(s) -
Jeune Hélène Le,
Jourdan François
Publication year - 1993
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903360209
Subject(s) - choline acetyltransferase , cholinergic , biology , olfactory bulb , ultrastructure , neuroscience , immunocytochemistry , axon , glomerulus , cholinergic neuron , sensory system , cholinergic fibers , olfactory system , acetylcholine , synaptic vesicle , anatomy , central nervous system , vesicle , endocrinology , biochemistry , membrane , kidney
The ultrastructural organization of cholinergic afferents to the rat olfactory bulb (OBI) was studied with the aid of choline acetyltransferase (ChAT) immunocytochemistry in electron microscopy. Particular attention has been paid to a subset of glomeruli characterized by a remarkably high density of cholinergic afferents. Numerous cholinergic terminals making symmetric or asymmetric synaptic contacts were observed in the periglomerular area. ChAT‐labelled terminals have a diameter ranging from 0.3 to 1.5 μm and contain numerous clear agranular vesicles. Axo‐somatic and axo‐dendritic contacts were both observed in contact with several types of target neurons. Three types of cholinoceptive, noncholinergic neurons could be identified: periglomerular cells, superficial short‐axon cells, and external tufted cells. Our results provide an anatomical substrate for the hypotheses concerning the complex effects of acetylcholine in the processing of sensory information in the olfactory bulb. © 1993 Wiley‐Liss, Inc.