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Developmental study of the gene expression for α and γ subunits of enolase in the rat brain by in situ hybridization histochemistry
Author(s) -
Watanabe Masahiko,
Nagamine Tomoaki,
Sakimura Kenji,
Takahashi Yasuo,
Kondo Hisatake
Publication year - 1993
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903270304
Subject(s) - enolase , in situ hybridization , biology , protein subunit , cerebellum , gene expression , immunohistochemistry , developmental profile , microbiology and biotechnology , messenger rna , gene , biochemistry , neuroscience , endocrinology , immunology
The gene expression for α and γ subunits of enolase, a dimeric enzyme in the glycolytic pathway, was examined in the developing brain of rats by in situ hybridization. The expression for the γ subunit of enolase was first detected in post‐mitotic neurons settled in the mantle zone at E13, and it increased progressively until the adult stage. Expression signals for the α subunit were discerned in two discrete regions showing different developmental changes: the signals in the proliferative ventricular zone were intense at E13 and decreased and eventually disappeared around birth, whereas the signals in the mantle zone persisted until the adult stage. In the adult brain, mRNAs for the α and γ subunits were expressed widely in neurons, resulting in almost similar temporal patterns in the brain except for the cerebellum. Expression levels of the α subunit in adult glial cells were below the detection threshold of the in situ hybridization analysis. These findings suggest that both α and γ enolase subunits participate in energy production in neurons of the mature brain and that marked changes in the subunit composition of enolase occur according to both neuron type and maturation. (c) 1993 Wiley‐Liss, Inc.