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Anatomy and fine structure of neurons in the deutocerebral projection pathway of the crayfish olfactory system
Author(s) -
Mellon De Forest,
Alones Vinessa,
Lawrence M. David
Publication year - 1992
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903210109
Subject(s) - crayfish , biology , postsynaptic potential , procambarus clarkii , anatomy , efferent , neuroscience , olfactory system , afferent , biochemistry , receptor , fishery
Golgi impregnation and neurobiotin injection were used to examine details of the neural pathways in the olfactory system of the freshwater crayfish, Procambarus clarkii . Deutocerebral projection neurons (globuli cells) were directly injected with neurobiotin. These neurons have dendritic arborizations in the ipsilateral olfactory and accessory lobes, and they project axons to the lateral protocerebrum, where they terminate in microglomeruli of the hemiellipsoid body. The axons of the deutocerebral projection neurons are readily impregnated by Golgi procedures, and they terminate as an expanded membranous knot about 5 μm in diameter. Electron microscopy on Golgi‐stained terminals has revealed that each knot makes several hundred synapses with small spine‐like or shaft‐like processes of postsynaptic neurons. Injection of neurobiotin into local interneurons of the hemi‐ellipsoid body and subsequent examination of stained preparations with the electron microscope reveals that these cells are a major postsynaptic target of the deutocerebral projection neurons. Furthermore, the local interneurons make extensive efferent synaptic connections with unidentified neurons in the terminal medulla. © 1992 Wiley‐Liss, Inc.