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Transmitter diversity in ganglion cells of the guinea pig gallbladder: An immunohistochemical study
Author(s) -
Talmage Erin K.,
Pouliot Wendy A.,
Cornbrooks Ellen B.,
Mawe Gary M.
Publication year - 1992
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903170104
Subject(s) - vasoactive intestinal peptide , substance p , medicine , neurotensin , endocrinology , somatostatin , biology , neuropeptide , neuropeptide y receptor , galanin , population , myenteric plexus , enkephalin , guinea pig , acetylcholine , gallbladder , immunohistochemistry , receptor , opioid , environmental health
Several neurotransmitters have been reported to exist in the ganglionated plexus of the guinea pig gallbladder. These include substance P, neuropeptide Y (NPY), calcitonin gene‐related peptide, vasoactive intestinal peptide (VIP), acetylcholine, norepinephrine, serotonin and dopamine. To determine which neuropeptides are intrinsic to gallbladder ganglia, we performed immunohistochemistry on colchicine‐treated preparations. In separate, single‐labeled preparations, a majority of neurons contained substance P‐, NPY‐, or somatostatin‐like immunoreactivity. In double‐labeled preparations, a large majority of the neurons that contained substance P‐like immunoreactivity also contained NPY‐like immunoreactivity and somatostatin‐like immunoreactivity. Immunoreactivity for VIP was present in a small percentage of the gallbladder neurons which did not contain substance P‐like immunoreactivity. Additional experiments were done to test for the presence of other compounds, known to exist in the neurons of the gut. Although immunoreactivity was found in control preparations of small intestine, the ganglionated plexus of the gallbladder lacked immunoreactivity for galanin, dynorphin, enkephalin, gastrin‐releasing peptide, or gamma‐aminobutyric acid. We conclude that ganglia of the guinea pig gallbladder contain at least two populations of neurons, based on transmitter phenotype. One of these populations appears to contain substance P, NPY, and somatostatin. Another population, which represents a small contingent of the total population of neurons, contains VIP.