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Altered expression of pp60 c‐src induced by peripheral nerve injury
Author(s) -
Ignelzi Michael A.,
Padilla Stephanie S.,
Warder Daryl E.,
Maness Patricia F.
Publication year - 1992
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903150205
Subject(s) - proto oncogene tyrosine protein kinase src , biology , growth cone , dorsal root ganglion , neurite , sciatic nerve , microbiology and biotechnology , anatomy , axon , spinal cord , neuroscience , signal transduction , biochemistry , in vitro
The normal src protein (pp60 c‐src ) is localized principally in the nerve growth cone of developing neurons and declines to low levels with synaptic maturation. To determine whether pp60 c‐src is reexpressed in regenerating axons, its expression was studied by immunoblotting and immunocytochemical analyses in adult chicken sciatic nerve following nerve crush injury. pp60 c‐src expression was found to increase during nerve repair with a temporal and spatial pattern consistent with a localization in regenerating axons. At the crush site, pp60 c‐src increased to maximal levels 7 days postinjury, increasing fivefold relative to 0 day nerve. In the nerve segment distal to the injury, the maximal increase in pp60 c‐src was sevenfold and occurred between 11 and 21 days postinjury. Immunoperoxidase staining revealed pp60 c‐src in regenerating axons and certain nonneuronal cells at the site of nerve repair. pp60 c‐src was induced in both motor and sensory neurons, as shown by increased pp60 c‐src immunoreactivity in their cell bodies located in the spinal cord and dorsal root ganglion. Phosphotyrosine‐modified proteins that were potential targets of pp60 c‐src increased following nerve crush, and were localized to outgrowing neurites as well as to nonneuronal cells. These results suggest that pp60 c‐src is a common component of cellular mechanisms regulating growth cone migration in both regenerating and developing axons.