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Mapping of glial fibrillary acidic protein‐immunoreactivity in the rat forebrain and mesencephalon by computerized image analysis
Author(s) -
Zilles Karl,
Hajós Ferenc,
Kálmán Mihály,
Schleicher Axel
Publication year - 1991
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903080303
Subject(s) - biology , glial fibrillary acidic protein , midbrain , interpeduncular nucleus , piriform cortex , olfactory bulb , forebrain , anatomy , globus pallidus , anterior olfactory nucleus , gfap stain , tegmentum , substantia nigra , lateral hypothalamus , neuroscience , olfactory tubercle , hippocampus , hypothalamus , central nervous system , basal ganglia , immunohistochemistry , dopamine , dopaminergic , immunology
Computer‐assisted image analysis was used to map the regional distribution of glial fibrillary acidic protein‐immunoreactive (GFAP‐IR) astrocytes in the rat forebrain, and mesencephalon. A complete survey of packing densities of GFAP‐IR structures was performed. Computer maps revealed high values in the outer and inner layers of the cortex, some hippocampal and olfactory bulb layers, prepiriform cortex, dorsal part of the caudate‐putamen, globus pallidus, lateral septum, reticular thalamic nucleus, lateral habenular nucleus, circumventricular organs, nuclei of the medial hypothalamus, substantia nigra, interpeduncular nucleus, and mamillary body. These correspond to regions of the embryonic pial and ventricular brain surfaces, which undergo developmental alterations including growth and various forms of internalization. From this we conclude that in the adult brain, astrocytes of high GFAP‐IR are derivatives of surface‐contact glia, whereas those located in areas having developed by the local thickening of the neural tube wall show reduced or no GFAP‐IR.