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Distribution of cells containing mRNA encoding cholecystokinin in the rat central nervous system
Author(s) -
Schiffmann S. N.,
Vanderhaeghen J.J.
Publication year - 1991
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903040206
Subject(s) - cholecystokinin , anterior olfactory nucleus , tegmentum , subiculum , parabrachial nucleus , biology , nucleus , thalamus , raphe nuclei , neuroscience , reticular formation , hippocampus , olfactory bulb , hippocampal formation , central nervous system , anatomy , olfactory tubercle , midbrain , dentate gyrus , serotonin , biochemistry , receptor , serotonergic
The distribution of cells containing mRNA encoding cholecystokinin was studied in the rat central nervous system by in situ hybridization histochemistry. Cholecystokinin mRNA containing neurons were considerably more numerous than the cholecystokinin‐like immunoreactive neurons detected by immunocytochemistry even after cholchicine pretreatment and appeared to be heavily, moderately, or lightly labeled. Such neurons were present in the olfactory bulb, olfactory nuclei, layers II–III and V–VI of the cerebral cortex, amygdaloïdnuclei, subiculum, hippocampus, claustrum, endopiriform nucleus, several hypothalamic nuclei, most of the thalamic nuclei, ventral tegmental area, substantia nigra, interfascicularis nucleus, linearis rostralis, central gray, Edinger‐Westphal nucleus, superior and inferior colliculi, parabrachial nucleus, reticular formation, raphe nuclei, and spinal trigeminal nucleus. This distribution partly confirmed and partly extended the previous immunohistochemical descriptions. Several brain areas such as the thalamus and the colliculi contain cholecystokinin mRNA but are devoid of perikarya exhibiting cholecystokinin‐like immunoreactivity. The cerebral cortex and the hippocampus present a far higher density of cholecystokinin mRNA containing cells, including pyramidal neurons, than of perikarya containing cholecystokinin‐like immunoreactivity. These results suggest that cholecystokinin or cholecystokinin‐related peptides could have a functional role in numerous cerebral pathways including long projections such as cortical or thalamic projections.