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GABA‐like immunoreactivity in a population of locust intersegmental interneurones and their inputs
Author(s) -
Watson Alan H. D.,
Laurent Gilles
Publication year - 1990
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903020408
Subject(s) - biology , lucifer yellow , horseradish peroxidase , locust , interneuron , immunocytochemistry , population , inhibitory postsynaptic potential , ganglion , neuroscience , gabaergic , anatomy , intracellular , microbiology and biotechnology , biochemistry , botany , enzyme , endocrinology , demography , sociology , gap junction
Intracellular labelling of locust intersegmental interneurones with lucifer yellow or horseradish peroxidase was carried out in combination with light and electron microscope immunocytochemistry by using an antibody raised against gamma amino butyric acid (GABA). Fifteen percent (four out of 27) of intracellularly stained interneurones showed GABA‐like immunoreactivity. This is in agreement with previous physiological observations that 20% of the interneurones in this population make inhibitory output conceptions in the metathoracic ganglion. GABA‐like immunoreactivity was also found in processes presynaptic to the interneurones in the mesothoracic ganglion. The presence of such immunoreactive inputs onto the intersegmental interneurones correlates well with physiological evidence that their receptive fields are in part shaped by direct input from GABA‐ergic spiking local interneurones.