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Long‐term survival and sprouting in culture by motoneurons isolated from the spinal cord of adult frogs
Author(s) -
Kuffler Damien P.
Publication year - 1990
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.903020405
Subject(s) - biology , spinal cord , sprouting , central nervous system , anatomy , bovine serum albumin , matrix (chemical analysis) , extracellular matrix , synapse , neuroscience , microbiology and biotechnology , immunology , botany , chemistry , chromatography
Motoneurons of adult frog spinal cord have been retrogradely labeled with the carbocyanine derivative diI. Spinal cords were then dissociated and the labeled motoneurons partially purified by centrifugation over a bovine serum albumin (BSA) density gradient. The resulting cell suspension was plated on a substrate of innervated muscle extracellular matrix (ECM) to which the motoneurons attached and extended processes. Labeled adult motoneurons survived for more than 4 weeks in a defined medium in the absence of added serum or growth factors. These cultures of adult motoneurons provide a favorable preparation for studying molecular factors that influence process outgrowth and synapse formation.