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Autoradiographic distribution of thyrotropin‐releasing hormone receptors in the african lungfish Protopterus annectens
Author(s) -
Pack Alison M.,
Caine S. Barak,
Winokur Andrew,
Manaker Scott,
Fishman Alfred P.
Publication year - 1989
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/cne.902870103
Subject(s) - lungfish , biology , diencephalon , cerebrum , endocrinology , medicine , receptor , spinal cord , brainstem , olfactory bulb , central nervous system , neuroscience , biochemistry , fishery , fish <actinopterygii>
We used quantitative autoradiography to examine the distribution of thyrotropin‐releasing hormone (TRH) receptors in the central nervous system (CNS) of the African lungfish Protopterus annectens. We found that the distribution of TRH receptors throughout the CNS of the lungfish was heterogeneous with the highest concentrations (500–800 fmol/mg protein) in the olfactory bulb and telencephalon, moderately high concentrations (200–500 fmol/mg protein) in the diencephalon, and moderate (50–200 fmol/mg protein) to low (<50 fmol/mg protein) concentrations in the brainstem and spinal cord. Except for the motor nuclei of the cranial nerves and spinal cord, TRH receptors were concentrated in the acellular regions. In the telencephalon and diencephalon, the receptor density was inversely related to cellular density. These results provide a neuroanatomic and neuropharmacologic basis for further investigations of TRH in the African lungfish.